Nucleotide Testing Requirements




4552 NEW ZEALAND GAZETTE No. 170

b) IMP, UMP and GMP must test positive for sodium phosphate

c) IMP, UMP, AMP, CMP and GMP must test positive for organic phosphate

  1. Assay (HPLC):

Optimum - not less than 96% (corrected for moisture content).

  1. IMP and GMP have a pH of a 1 in 20 solution: between 7.0 and 8.5

  2. Clarity and colour of solution:

500mg/10mL H₂O for IMP: is colourless and shows only a trace of turbidity

100mg/10mL H₂O for GMP: is colourless and shows only a trace of turbidity

  1. Moisture
Nucleotide Moisture
Inosine - 5' monophosphate disodium salt Not more than 28.5%: Karl Fischer
Uridine - 5' monophosphate disodium salt Not more than 26.0%: Karl Fischer
Guanosine - 5' monophosphate disodium salt Loss in drying
- not more than 25%
(4 hrs @ 120°C)
Cytidine - 5' monophosphate Not more than 6.0%: Loss in drying (4 hrs @ 120°C)
Adenosine- 5' monophosphate Not more than 6.0%: Loss in drying (4 hrs @ 120°C)
  1. Impurities - all nucleotides
Impurity Nucleotide
Amino acids: negative IMP, GMP
Ammonium salts: negative IMP, GMP
Arsenic: not more than 2ppm IMP, UMP, AMP, CMP, GMP
Heavy metals: not more than 10ppm IMP, UMP, AMP, CMP, GMP


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Online Sources for this page:

VUW Te Waharoa PDF NZ Gazette 2000, No 170


Gazette.govt.nz PDF NZ Gazette 2000, No 170





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🏥 Testing Requirements for Nucleotides (continued from previous page)

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Nucleotides, Testing, Sodium phosphate, Organic phosphate, HPLC, pH, Clarity, Colour, Moisture, Impurities